rabbit polyclonal antibody against p ampk  (Cell Signaling Technology Inc)

Journal: Biological & pharmaceutical bulletin

Article Title: Polydatin Improves Glucose and Lipid Metabolisms in Insulin-Resistant HepG2 Cells through the AMPK Pathway.

doi: 10.1248/bpb.b17-01027

Figure Lengend Snippet: Fig. 2. The Effects of 100 nM Insulin on the Phosphorylation Levels of Akt (A), AMPK (B) and Nuclear and Cytoplasm Protein Levels of SREBP-1c (C) of HepG2 Cell

Article Snippet: In the wake of washing, the layers were brooded overnight at 4°C with one of these primary antibodies: rabbit polyclonal antibodies against p-AMPK (Thr172; Cell Signaling Technology, Cat. No. 2535, U.S.A.), P-ACC (Ser79; Cell Signaling Technology, Cat. No. 11818), AMPK (Cell Signaling Technology, Cat. No. 5831), ACC (Cell Signaling Technology, Cat. No. 3676), P-Akt (ser473, Cell Signaling Technology; Cat. No. 4060), P-glycogen synthase kinase (GSK)-3β (ser9; Cell Signaling Technology, Cat. No. 5558), GSK-3β (1 : 1000; Cell Signaling Technology, Cat. No. 9315), and SREBP-1c (1 : 300; Santa Cruz Biotechnology, Cat. No. sc-17755, U.S.A.); rabbit monoclonal immune response against low-density lipoprotein receptor (LDLR) (1 : 1000; Proteintech, Cat. No. 10785-1-AP, U.S.A.) and Akt (pan; 1 : 1000; Cell Signaling Technology, Cat. No. 4691); mouse monoclonal immunizer against Tubulin (1 : 10000; Sigma), Actin (1 : 1000; Beyotime) and Histone 1.4 (1 : 1000; Sigma).

Techniques: Phospho-proteomics

Journal: Biological & pharmaceutical bulletin

Article Title: Polydatin Improves Glucose and Lipid Metabolisms in Insulin-Resistant HepG2 Cells through the AMPK Pathway.

doi: 10.1248/bpb.b17-01027

Figure Lengend Snippet: Fig. 5. The Effects of Polydatin on the Phosphorylation Levels of AMPK (A), Akt (B) and GSK-3β (C) of Insulin-Resistant HepG2 Cells

Article Snippet: In the wake of washing, the layers were brooded overnight at 4°C with one of these primary antibodies: rabbit polyclonal antibodies against p-AMPK (Thr172; Cell Signaling Technology, Cat. No. 2535, U.S.A.), P-ACC (Ser79; Cell Signaling Technology, Cat. No. 11818), AMPK (Cell Signaling Technology, Cat. No. 5831), ACC (Cell Signaling Technology, Cat. No. 3676), P-Akt (ser473, Cell Signaling Technology; Cat. No. 4060), P-glycogen synthase kinase (GSK)-3β (ser9; Cell Signaling Technology, Cat. No. 5558), GSK-3β (1 : 1000; Cell Signaling Technology, Cat. No. 9315), and SREBP-1c (1 : 300; Santa Cruz Biotechnology, Cat. No. sc-17755, U.S.A.); rabbit monoclonal immune response against low-density lipoprotein receptor (LDLR) (1 : 1000; Proteintech, Cat. No. 10785-1-AP, U.S.A.) and Akt (pan; 1 : 1000; Cell Signaling Technology, Cat. No. 4691); mouse monoclonal immunizer against Tubulin (1 : 10000; Sigma), Actin (1 : 1000; Beyotime) and Histone 1.4 (1 : 1000; Sigma).

Techniques: Phospho-proteomics